Research Paper Volume 15, Issue 23 pp 13980—13997

Figure 6. LncRNA GPRC5D-AS1 regulated cell viability and cell apoptosis by miR-520d-5p. (A) Cell viability was assessed by CCK-8 assay. 15 mM Dex was added in HSMM to establish atrophy cell model (control group). Empty plasmid (NC group), GPRC5D-AS1-OE (lncRNA-OE group), GPRC5D-AS1-OE + miR-520d-5p mimic (lncRNA-OE + mimic group), miRNA inhibitor control (inhibitor NC group), miR-520d-5p inhibitor (miRNA inhibitor group) or MYOD1-OE plasmid (mRNA-OE group) was transfected into atrophy cell model and incubated for 24 h, 48 h and 72 h. ^*P < 0.05 compared with control group; ^#P < 0.05 compared with NC group; ^&P < 0.05, ^&&P < 0.01 compared with lncRNA-OE group. (B) Cell apoptosis was assessed by flow cytometry. Groups were set as previously mentioned. Six different plasmids were transfected into atrophy cell model and incubated for 48 h. (C) Quantitative analysis of cell apoptosis. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with NC group; ^&P < 0.05, ^&&P < 0.01 compared with lncRNA-OE group; ^%P < 0.05, ^%%P < 0.01 compared with inhibitor NC group.