Research Paper Volume 15, Issue 23 pp 13980—13997

Figure 5. LncRNA GPRC5D-AS1 interacted with miR-520d-5p to promote myoblast proliferation. (A, B) qRT-PCR analyzed gene expression of GPRC5D-AS1, miR-520d-5p, MyoD1, MyoG, Mef2c, Myf5 and Wnt5a. 15 mM Dex was added in human skeletal muscle myoblasts to establish atrophy cell model (control group). Empty plasmid (NC group), GPRC5D-AS1-OE (lncRNA-OE group), GPRC5D-AS1-OE + miR-520d-5p mimic (lncRNA-OE + mimic group), miRNA inhibitor control (inhibitor NC group), miR-520d-5p inhibitor (miRNA inhibitor group) or MYOD1-OE plasmid (mRNA-OE group) was transfected into atrophy cell model and incubated for 48 h. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with NC group; ^&P < 0.05, ^&&P < 0.01 compared with lncRNA-OE group; ^%P < 0.05, ^%%P < 0.01 compared with inhibitor NC group.