Research Paper Volume 15, Issue 23 pp 13961—13979

Figure 8. CTSV inhibits T cell activity in vitro. (A–K) Co-cultured the indicated stable A549 and NCI-H1975 cells with PBMCs for 48 h, then the remaining cells were fixed and dyed with 1% crystal violet. Western Blotting analysis of the remaining A549 and NCI-H1975 cells using anti-cleaved casepase3/−7 antibodies (B, E, I, J). Caspase 3/7 activity was measured using the Caspase-Glo 3/7 assay. Each bar represents the mean ± SEM (C, F, K). (L, M) The indicated stable A549 and NCI-H1975 cells were pre-treated with CTSV antibodies and then co-cultured with PBMCs for 48 h. Next, the remaining cells were fixed and dyed with 1% crystal violet. (N, O) Western Blotting analysis of the remaining A549 and NCI-H1975 cells from (L, M) using anti-cleaved casepase3/−7 antibody. (P) Caspase 3/−7 activity was measured using the Caspase-Glo 3/7 assay. ^**P-value < 0.01 and ^***P-value < 0.001.