Research Paper Volume 15, Issue 23 pp 13920—13943

Figure 7. CDKN1A was regulated by hsa-miR-300 via both GADD45B and TP53. (A–F) In A375 cells, the CDKN1A expression after transfecting with miR-300 mimics and inhibitors, simultaneously transfecting with TP53 and GADD45B expression or knockdown vector. There was a blank control group and a negative control group. (-) represents gene interference and (+) represents gene expression. (G, H) In A375 cells, the cell cycle progress alteration after transfecting with miR-300 mimics and inhibitors, simultaneously transfecting with TP53 and GADD45B expression or knockdown vector. The proportion of cells in each group at different stages was calculated by the corresponding histogram. Blue represents the G1 phase, green represents the S phase, and red represents the G2 phase. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.