Research Paper Volume 16, Issue 5 pp 4138—4148

Figure 8. Silencing of KLF2 abolished the protective effects of Azilsartan against ANG/HS-induced aggravation of endothelial permeability in HrGECs. HrGEC monolayer was transduced with lentiviral KLF2 shRNA, followed by stimulation with ANG/HS with or without Azilsartan (5 μM) for 24 hours. (A) Western blot revealed successful knockdown of KLF2; (B) mRNA level of occludin; (C) Fluorescence intensity of FITC-dextran (n=6, ^^, P<0.01 vs. vehicle group; **, P<0.01 vs. ANG/HS group; ##, P<0.01 vs. ANG/HS+ Azilsartan group).