Research Paper Volume 15, Issue 23 pp 13753—13775
PBRM1 mutation and WDR72 expression as potential combinatorial biomarker for predicting the response to Nivolumab in patients with ccRCC: a tumor marker prognostic study
Figure 4. Effects of WDR72 gene expression on fatty acid beta-oxidation and proliferation of ccRCC cells. (A) Western blot analysis showed the protein expression of WDR72 and CPT1A (the critical enzyme of FAO after WDR72 overexpression in 786-O and ACHN cells. (B, C) FAO and ATP level FAO after WDR72 overexpression in 786-O and ACHN cells. (D, E) CCK8 showed the proliferative capacity of 786-O and ACHN cells after WDR72 overexpression. Abbreviations: WDR72, WD repeat-containing protein 72; FAO, fatty acid oxidation; CPT1A, carnitine palmitoyltransferase 1A; ATP, adenosine triphosphate; OD, optical density; * P <0.05, ** P <0.01.