Research Paper Volume 15, Issue 22 pp 13329—13344

Figure 4. circSLCO1B7 functions as miR-556-3p sponge. (A) Bioinformatics analysis predicted the miRNAs that circSLCO1B7 might competitively bind to miRNAs. (B) RIP experiments were performed to identify the miRNAs to which circSLCO1B7 might competitively bind. (C, D) The luciferase activity of circ_0025583-WT or circ_0025583-MUT in the miR-556-3p mimic or NC in SK-HEP-1 and PLC/PRF/5 cells was shown. (E) The expression of miR-556-3p in HCC tissues was detected by qRT–PCR. (F) The expression of miR-556-3p was negatively related to circSLCO1B7 in HCC tissues. (G) The survival analysis of miR-556-3p was downloaded from Kaplan–Meier plotter (n = 166). (H) The transfection efficiency of the miR-556-3p mimic and inhibitor was measured by qRT–PCR. All data are presented as the means ± SD. ^*P < 0.05, ^**P < 0.01.