Research Paper Volume 15, Issue 22 pp 13329—13344

Figure 2. Overexpression of circSLCO1B7 inhibited the progression of HCC cells. (A) qRT–PCR analysis of the expression of circSLCO1B7 in HCC cells. (B) The CCK-8 assay was used to detect the proliferation ability of transfected SK-HEP-1 and PLC/PRF/5 cells. (C) The migration and invasion abilities of transfected SK-HEP-1 and PLC/PRF/5 cells were measured by Transwell assay. (D) A wound healing assay was performed to detect the migration levels of transfected SK-HEP-1 and PLC/PRF/5 cells. (E, F) After overexpression in SK-HEP-1 and PLC/PRF/5 cells, the apoptosis rate (E) and distribution of the cell cycle (F) were analysed by flow cytometry and compared to the vector group. All data are presented as the means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001.