Research Paper Volume 15, Issue 22 pp 13194—13212
Electroacupuncture ameliorates AOM/DSS-induced mice colorectal cancer by inhibiting inflammation and promoting autophagy via the SIRT1/miR-215/Atg14 axis
Figure 5. Atg14 is a direct target of miR-215. (A) RT-qPCR analysis of Atg gene expression after SIRT1 overexpression. (B) Schematic illustration of the predicted miR-215-5p docking sequences for Atg14. (C) The luciferase reporter activity of Luc-ATG14-WT/MUT was detected by dual luciferase reporter assay. (D, E) Transfection of mimic-miR-215-5p or inhibitor-miR-215-5p in SW480 and HCT116 cells to evaluate Atg14 proteins (D) and mRNA (E) levels. Data are presented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the control group.