Research Paper Volume 15, Issue 22 pp 13176—13193
Investigating EGF and PAG1 as necroptosis-related biomarkers for diabetic nephropathy: an in silico and in vitro validation study
Figure 6. Activation of necroptotic and necroptosis-related biomarkers in HK-2 cells stimulated by high glucose. (A) Western blot assays were used to detect the expression levels of necroptotic proteins in HK-2 cells under different treatments. (B) Bar charts show the grey values obtained from the western blots shown in (A). (C) Reverse transcription quantitative PCR analysis was used to quantify the expression levels of necroptotic gene markers in HK-2 cells treated with NG, NG+M and HG, respectively. The protein and mRNA expression levels of EGF, PAG1 and ZFP36 were detected by western blot analysis (D, E) and reverse transcription quantitative PCR (F) in HK-cells when treated with NG, NG+M and HG. (#P < 0.05, ##P < 0.01, compared with NG group). Abbreviations: NG: normal glucose; NG+M: NG + Hyperosmotic medium; HG: high glucose; DN: diabetic nephropathy.