Research Paper Volume 15, Issue 22 pp 13163—13175

Figure 2. Hypoxia promotes exosome secretion from chondrosarcoma cells that induce the polarization of M2 macrophages. (A) qRT-PCR was used to detect the expression of CD68 (macrophage marker). (B) Macrophages were treated with SW1353-N-exo, SW1353-H-exo, or control (PBS and IL-4). After 48 h, qRT-PCR was applied using primers for M2 surface markers (CD163 and CD206) and an M1 surface marker (CD86). The group treated with IL-4 was used as a positive control. (C, D) Western blot analysis was used to detect the expression of M2 surface marker CD206. All replicate number ≧ 3. ^#p < 0.05 compared with the THP-1 group; ^*p < 0.05 compared with the SW1353-N-exo group.