Research Paper Volume 15, Issue 21 pp 11764—11781

Figure 4. A double combination, of doxycycline plus azithromycin, extends life span of C. elegans. (A) Survival curve at different concentrations of antibiotics: untreated control (FUdR-only) (blue), Azi plus Doxy (1 μM each) (red), vitamin C alone (250 μM) (green) and triple combination (Azi plus Doxy plus vitamin C) (purple). Statistical analysis was performed using Log-rank (Mantel-Cox), ^**p < 0.01 (control vs. Doxy plus Azi). (B) Median survival and maximal lifespan are represented. Experiments were performed in triplicate, with 15 worms for each replicate.