Research Paper Volume 15, Issue 22 pp 12873—12889

Figure 5. Role of ADSL signaling in Dicer-mediated DTIC resistance and stemness in melanoma cells. (A) Western blotting results and (B) qRT-PCR results indicating the levels of Dicer and ADSL in Dicer-silenced A375 cells transfected with ADSL overexpression plasmids. (C) Cell viability (assessed through the MTT assay) 48 and 72 h after treatment with DTIC. (D) Expression levels of Oct-4, Nanog, SOX2, and KLF4 mRNA (measured through QRT-PCR). (E) Western blotting and qRT-PCR results indicating the levels of Dicer and ADSL in Dicer-overexpressing A2508 cells transfected with ADSL-silenced plasmids. (F, G) Cell viability (assessed through the MTT assay) and the expression levels of various cancer stem cell markers (measured through qRT-PCR) upon treatment with varying concentrations of DTIC: Oct-4, Nanog, SOX2, and KLF4. Data are presented in terms of the mean ± standard error of the mean of three independent experiments, each performed in triplicate. ^**P < 0.01 and ^***P < 0.001 (Student’s t test).