Research Paper Volume 15, Issue 22 pp 12873—12889

Figure 1. Association of Dicer expression with dacarbazine (DTIC) treatment response and cancer stemness in melanoma cells. (A) Viability of A375 and A2508 melanoma cells treated with varying concentrations of DTIC for 48 and 72 h. (B) Western blotting results indicating the expression levels of Dicer in A375 and A2508 melanoma cells treated with varying concentrations of DTIC. (C) Western blotting results indicating the expression levels of Dicer in A375 and Dicer-silenced (shDicer #1 and #2) cells. (D) Viability of melanoma cells treated with varying concentrations of DTIC (measured through the MTT assay). (E) Expression levels of Oct-4, Nanog, SOX2, and KLF4 in Dicer-silenced (shDicer #1 and #2) cells (evaluated through qRT-PCR and Western blotting). Data are presented in terms of the mean ± standard error of the mean of three independent experiments, each performed in triplicate. ^*P < 0.05 and ^**P < 0.01 (Student’s t test).