Figure 4. Centrosome amplification in ESCC tissues and paired analysis with KIFC1 expression levels. (A) Centrosome staining in ESCC tissues and adjacent tissues, centrosomes marked using anti-gamma-tubulin antibodies (green) and DNA stained with DAPI (blue); (B) Analysis of centrosome amplification phenomena in relation to tumor grade; (C) Analysis of the relationship between KIFC1 expression levels and rates of centrosome amplification. n = 30, *< 0.05, **< 0.01.