Research Paper Volume 15, Issue 21 pp 12633—12650

Figure 3. Overexpression of KIFC1 promotes centrosome amplification and pseudo-bipolar division. (A) Schematic illustration of centrosome amplification in KIFC1 overexpression group and control group, where centrosomes were labeled with gama-tubulin antibody (green) and DNA was stained with DAPI (blue); (B) Western blot analysis the overexpression of KIFC1; (C) Statistics of centrosome amplification rate in cells after KIFC1 overexpression; (D) Statistics of the ways cells enter mid and late stages of mitosis; (E) Schematic illustrations of bipolar division, pseudo-bipolar division, and multi-polar division in cells, where centrosomes were labeled with gama-tubulin antibody (green) and DNA was stained with DAPI (blue). n = 3, ^**< 0.01.