Research Paper Volume 15, Issue 21 pp 12537—12550
Cyclosporin A-mediated translocation of HuR improves MTX-induced cognitive impairment in a mouse model via NCOA4-mediated ferritinophagy
Figure 5. CsA alleviated neuronal apoptosis in MTX-excited HT22 cells through HuR. (A–D) Representative results on apoptotic cells in the Control, MTX, MTX+CsA+NC-siRNA, and MTX+CsA+HuR-siRNA groups, as evaluated using flow cytometry. (E) Flow cytometry analysis of HT22 cells in the Control, MTX, MTX+CsA+NC-siRNA, and MTX+CsA+HuR-siRNA groups. (n=3 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (F) HT22 cell, as captured after co-staining with HuR and NCOA4. In the representative images, HuR and NCOA4 are shown in red and green, respectively. Representative micrographs: ×400 magnification. (G) HR22 cell lysis immunoprecipitation with anti-HuR antibody or control IgG.