Research Paper Volume 15, Issue 21 pp 12537—12550

Figure 1. Effects of CsA on MTX-induced cognitive impairment in mice. (A) The total distance travelled during the initial 5 min of exposure to the training box in the open field test. (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (B) The time spent in the center of the open field during 5 min of exposure to the training box in the open field test. (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (C) The total percentage of freezing during the 5 min of contextual fear conditioning test (test phase of the FCT). (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (D) The recognition index of the novel object in the NOR. (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (E) Movement tracks showing 5 min of open field exploration by mice. (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (F) Neuronal apoptosis in the CA1, CA3 and DG regions of the hippocampus, as determined using Tunnel staining. Representative micrographs: ×400 magnification. (n=6 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. (G) The percentage of damaged neurons. The data present the means ± standard error of the mean. (n=5 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group.