Research Paper Volume 15, Issue 21 pp 12497—12512
Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis
Figure 3. FA alleviates aging-induced oocyte mitochondrial dysfunction. (A) Oocytes were stained with MitoTracker Red CMXRos and JC-1 dyes to detect intracellular mitochondrial activity and MMP levels. Scale bar: 100 μm. R = 3. (B, C) Relative intracellular levels of active mitochondria and MMP in bovine oocytes from the three groups (Fresh, Aged, and Aged + FA group). (D) Relative intracellular ATP levels in bovine oocytes from the three groups (Fresh, Aged, and Aged + FA group). R = 6. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences.