Figure 2. lncRNA HOXC-AS2 was upregulated in human hypopharyngeal carcinoma tissues and localized mainly in the cytoplasm. (A) Representative images of HOXC-AS2 expression in hypopharyngeal carcinoma tissues, as verified by FISH; the scale bars represent 500 μm and 20 μm, respectively. (B) Representative image of HOXC-AS2 expression in normal hypopharyngeal carcinoma-adjacent tissues, as verified by FISH; the scale bars represent 500 μm and 20 μm, respectively. (C) The intracellular localization of HOXC-AS2 was determined by RNA FISH in the FADU cell line. (D) The intracellular localization of HOXC-AS2 was determined by RNA FISH in the Detroit-562 cell line. (E) The nuclear and cytoplasmic distribution of HOXC-AS2 in the FADU cell line was evaluated by qRT-PCR.