Research Paper Volume 15, Issue 21 pp 12330—12368

Figure 12. Correlations of risk score with clinical characteristics and biological pathways. (A) Correlations of risk score with clinical characteristics. Risk score was significantly correlated with prognostic cluster (p = 0), T staging (p = 1e-04), N staging (p = 1e-04), survival status (p = 0) and cancer status (p = 0.0023). (B) Comparisons of risk scores for patients between two clusters. Risk score in the cluster 1 was significantly higher than that in the cluster 2. ****p < 0.0001. (C) Comparisons of risk scores for patients between four N stages. Risk scores of patients with stage N0 were significantly lower than those with stage N1 and those with stage N2. **p < 0.01 and ***p < 0.001. (D) Comparisons of risk scores for patients between three M stages. The risk scores had no significant differences between three M stages. (E) Comparison of risk scores for patients between <= 60 and > 60 age subgroups. The risk scores had no significant differences between two age subgroups. (F) Comparisons of risk scores for patients between four T stages. Risk scores of patients with stage T1 were significantly lower than those with stage T2 and those with stage T3. ****p < 0.0001. (G) Comparison of risk scores for patients between two survival statuses. The risk scores for the living LUAD patients were significantly lower than those for dead patients. ****p < 0.0001. (H) Comparisons of risk scores for patients between three cancer statuses. The risk scores for patients with tumor free were significantly lower than those with discrepancy tumor. *p < 0.05 and ***p < 0.001. (I) Gene set variation analysis. At the correlation of risk score with KEGG pathway > 0.4 or < -0.4 and p < 0.001, thirteen KEGG pathways were positively correlated with risk score. (J) Correlations of risk score with KEGG pathways. There was a strong positive correlation between risk score and 13 KEGG pathways. ***p < 0.001. (K) Top 5 KEGG pathways enriched in the high-risk group. Five KEGG pathways were separately cell cycle, DNA replication, homologous recombination, oocyte meiosis and P53 signaling pathway. (L) Top 5 KEGG pathways enriched in the low-risk group. Five KEGG pathways were separately ALPHA linolenic acid metabolism, cell adhesion molecules (CAMs), Fc epsilon RI signaling pathway, long term depression and viral myocarditis.