Research Paper Volume 15, Issue 22 pp 12723—12737

Figure 1. Synthesis of Tat-HSP10 and HSP10 proteins, their delivery into the hippocampus, and their effects on mitochondrial marker proteins. (A) Schematic diagrams of vectors encoding Tat-HSP10 and HSP10 are shown, and the expression is visualized and confirmed using Coomassie Brilliant Blue staining and western blotting for His-Tag. Clear bands are detected. Tat-HSP10 protein is detected at a slightly higher molecular weight compared to HSP10. (B) Delivery of Tat-HSP10 and HSP10 is visualized by immunohistochemical staining for His-Tag. His-Tag immunoreactive structures are abundantly detected in the Tat-HSP10-treated group, not in the HSP10-treated group. HSP, Heat shock protein; His, histidine. (C) Protein expressions such as His-Tag, Sirt3, and P16 are assessed by western blotting. His-Tag protein is highly expressed in the Tat-HSP10-treated group of adult and aged mice, but the protein level is significantly low in the aged group compared to that in the adult group. Sirt3 protein levels are increased in Tat-HSP10 treated groups of both the adult and aged groups. P16 levels show significantly higher levels in aged mice than in adult ones, and the protein level is significantly decreased in the aged group compared to the adult group. Data are represented as the mean ± SD (n = 5 each group; analyzed by one-way or two-way ANOVA test followed by Tukey’s post hoc test, ^aP < 0.05, vs. adult group; ^bP < 0.05, vs. control group; ^cP < 0.05, vs. HSP10-treated group). Abbreviations: HSP: Heat shock protein; Sirt: sirtuin; ANOVA: Analysis of Variance; SD: standard deviation.