Research Paper Volume 15, Issue 21 pp 12296—12313
A systematic framework for identifying prognostic necroptosis-related lncRNAs and verification of lncRNA CRNDE/miR-23b-3p/IDH1 regulatory axis in glioma
Figure 10. The expression of lncRNA CRNDE, IDH1 and miR-23b-3p and the function of lncRNA CRNDE in glioma cell lines. (A) LncRNA CRNDE expression in different glioma cell lines (U-118MG, U251 and U87) compared to normal astrocytes (NHA) were investigated by qRT-PCR. (B) miR-23b-3p expression in different glioma cell lines (U-118MG, U251 and U87) compared to normal astrocytes (NHA) were estimated by qRT-PCR. (C) IDH1 expression in different glioma cell lines (U-118MG, U251 and U87) compared to normal astrocytes (NHA) were estimated by qRT-PCR. (D) The efficiency of si-CRNDE. (E, F) CCK-8 assays in U-118MG and U251 cells. (G, H) Wound healing assays in U-118MG and U251 cells. *p<0.05; **p<0.01; ***p<0.001.