Research Paper Volume 15, Issue 21 pp 12251—12263

Figure 2. Cytotoxicity of PPI on HepG2/S and Huh-7/S cells. (A) HepG2/S and Huh-7/S cells were treated with various concentrations of PPI (ranging from 0 to 8 μM) for 24, 48, and 72 h. Cell viability was assessed using the CCK8 assay. The data are presented as means ± SD of three independent experiments. Statistical analysis was performed, and P-values less than 0.05 and less than 0.01 were considered significant when compared to control cells. (B) The effect of PPI (ranging from 0 to 8 μM) on SR-HCC cell viability was evaluated by cell counting assay after exposing the cells for 24, 48, and 72 h. (C) Clone formation assays were performed to examine cell vitality after PPI treatment in SR-HCC. The inhibitory effect of PPI on colony formation was evaluated by treating SR-HCC cells with various concentrations of PPI (ranging from 0 to 8 μM) for 2 weeks, while HCC cells were treated without PPI. Surviving colonies with more than 10 cells were counted. Bar graphs were used to represent the number of clones formed by HCC and SR-HCC cells after various treatments, with statistical significance indicated by asterisks (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).