Research Paper Volume 15, Issue 21 pp 12171—12191
CENPB promotes the proliferation of hepatocellular carcinoma and is directly regulated by miR-29a
Figure 5. CENPB knockdown inhibited the proliferation and migration capacities of HCC cells. (A) Expression of CENPB was upregulated in HCC cell lines compared to normal liver cells. (B, C) Transfection of shCENPB resulted in a decrease in CENPB mRNA levels in Hep3B (B) and MHCC97 (C) cell lines. (D) Western blot assay confirmed the inhibitory effects of shCENPB#3 on CENPB protein expression in Hep3B and MHCC97 cells. (E, F) CCK-8 assays demonstrated that knockdown of CENPB suppressed the viability of Hep3B (E) and MHCC97 (F) cells. (G, H) Transwell assays showed representative images and quantified analysis of cell invasion in Hep3B (G) and MHCC97 (H) cells transfected with shCtrl or shCENPB#3. *P < 0.05, **P < 0.01, ***P < 0.001. ns: not statistically significant.