Research Paper Volume 15, Issue 21 pp 12120—12135

Figure 7. USP14 activated the NF-κB pathway and mediated EMT. (A) USP14 overexpression plasmids and/or miR-124-3p mimics were transfected into HEC-1-B cells, and the profiles of I-κB and NF-κB in EC cells were examined by WB. ^** and ^***represent P < 0.01 and P < 0.001, (vs. vector). ^&&&indicates P < 0.001 (vs. USP14+miR-NC). (B) The levels of I-κB and NF-κB in tumors were detected by WB, ^**indicates P < 0.01, vs. Vector. (C) USP14-overexpressing cells were treated with BAY-1170829 (2 μM) to test the colony formation of HEC-1-B cells. (D) FCM was used to track EC apoptosis. (E) The protein profiles of Bcl-2, Bax, and Cyclin D1 were monitored by WB. (F) The levels of E-cadherin, Vimentin, Snail1, ZEB1, and Slug were compared by WB. ^** and ^***represent P < 0.01 and P < 0.001, respectively (vs. USP14+vehicle). N = 3.