Figure 2. Both 32P isotope injection and skin path significantly inhibited TGF-β/Smad signaling pathway. (A) The protein expression of TGF-β/Smad signaling pathway was measured with western blotting (n=3); (B) The mRNA levels of HIF-1α and TGF-β/Smad signaling pathway were analyzed (n=3); (C) The protein levels of Coll1α1 and Coll3α1 were analyzed (n=3). * indicates p <0.05.