Research Paper Volume 15, Issue 21 pp 11970—11984

Figure 4. Investigation and validation of the hub genes associated with EMT. (A–D) Four genes, including C1QTNF6, DLGAP5, HMMR, and PLEK2, had a significant survival relevance for OS in the TCGA cohort (log-rank test; P < 0.01). (E–H) Four genes, including C1QTNF6, DLGAP5, HMMR, and PLEK2, had a significant survival relevance for PFS in the GSE50081 cohort (log-rank test; P < 0.01). (I–L) Consistent with the above findings, the expression of the four genes was enhanced in tumor tissues compared with normal tissues using RNA-seq from the TCGA cohort (P < 0.001). (M–P) DLGAP5 and HMMR were significantly upregulated in the lung metastatic foci as compared with non-metastatic lung foci, while C1QTNF6 and PLEK2 were not. (Q–T) C1QTNF6, DLGAP5, HMMR, and PLEK2 were also significantly upregulated in the cancerous tissues as compared with matched normal tissues using RT-PCR for 32 pairs of LUAD tissues and matched normal tissues from our hospital (P < 0.001).