Research Paper Volume 15, Issue 21 pp 11940—11969

Figure 11. Effects of THSD7A gene deletion on gastric cancer cell proliferation and invasion. (A) The results of the qRT-PCR analysis of THSD7A expression in normal gastric cells and gastric cancer cells. (B) In HGC-27 cells, THSD7A expression is downregulated in the shRNA group, with the THSD7A-shRNA-1 group being downregulated most significantly. (C) CCK-8 assay results showing the proliferation and viability of HGC-27 cells following THSD7A knockout. (D, E) Wound-healing assay results showing the migration ability of HGC-27 cells following THSD7A knockout (scale bar = 20 μm). (F, G) Transwell assay results showing the invasion and migration abilities of HGC-27 cells in the NC-shRNA and THSD7A-shRNA groups (scale bar = 10 μm). (H, I) Colony formation assay results comparing the number of clones of HGC-27 cells between the NC-shRNA and THSD7A-shRNA groups (*: P<0.05, **: P<0.01, ***: P<0.001, ****:P<0.0001, ns: P>0.05. Error bars represent standard deviation). (GES-1 and NS-shRNA are the control group).