Research Paper Volume 15, Issue 20 pp 11639—11653

Figure 6. Alisol A reduced neuronal apoptosis in the hippocampus by downregulating the BAX/Bcl-2 ratio and activating the AKT/GSK3β pathway after CI. (A) Representative image of NeuN in the hippocampal CA1 region by using immunofluorescence staining. 200×, scale bar is 100 μm. (B) Quantitative analysis of NeuN fluorescence intensity, n = 3. (C) The neuronal morphology of the hippocampal CA1 region was observed by Nissl staining. 400×, scale bar is 50 μm. Red arrows show the morphology of neurons in different groups. (D) Quantitative analysis of intact neuronal cells in the hippocampal CA1 region, n = 3. (E) Western blot showing the expression of BAX and Bcl-2 and quantitative analysis of the BAX/Bcl-2 ratio. (F) Western blot showing the expression of p-AKT and AKT and quantitative analysis of the ratio of p-AKT to AKT. (G) Western blot showing the expression of p-GSK3β and GSK3β and quantitative analysis of the ratio of p-GSK3β to GSK3β, n = 4. Data are shown as the mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the sham group, ^#P < 0.05, ^###P < 0.001 compared with the CI group, ^@P < 0.05, ^@@P < 0.01 compared with the CI+AA group.