Research Paper Volume 15, Issue 21 pp 11845—11859

Figure 7. CAP relieved DOX-induced apoptosis of cardiomyocytes by regulating PI3K-Akt signaling pathway. (A) KEGG pathway enrichment plots for DOX group and CAP+DOX group 24 hours after injection of DOX (n = 3). (B) Quantitative analysis of heart tissue collected from four groups of mice after injection of saline or CAP or DOX or CAP pretreatment followed by DOX treatment, using immunoblotting (n = 3). (C) Quantitative and analytical immunoblotting for H9C2 cells treated with 2.5 µM DMSO, DOX or 0.4 µM CAP+2.5 µM DOX for 12 hours (n = 3). (D) Ratio of the BCL2 and BAX proteins in mouse myocardial tissue (top) and in H9C2 cells (bottom), used to indicate risk of apoptosis (n = 3). Data are shown as the mean ± SEM. Statistical significance was determined using two-tailed student’s t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.