Research Paper Volume 15, Issue 21 pp 11845—11859

Figure 5. CAP inhibited DOX-induced ferroptosis in H9C2 cells. (A) H9C2 cells were treated with 2.5 µM DMSO or DOX or varying concentrations of CAP+ 2.5 µM DOX for 6 hours, followed by measurement of cell viability using cck-8 assay (n = 5). (B) H9C2 cells were treated with 2.5 µM DMSO or DOX or 0.4 µM CAP+ 2.5 µM DOX for 6 hours, followed by measurement of cell toxicity using LOH assay (n = 5). (C) H9C2 cells were treated as described previously for 6 hours, followed by measurement of intracellular GSH/GSSG levels (n = 5). (D) H9C2 cells were treated with 2.5 µM DMSO or DOX or 0.4 µM CAP + 2.5 µM DOX for 24 hours, and then observed under bright field (BF) and fluorescence modes for measurement of intracellular ROS levels (top) and cell death(bottom) (n = 3). Scale: 20 µM Data are shown as the mean ± SEM. Statistical significance was determined using two-tailed student’s t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0001.