Research Paper Volume 15, Issue 21 pp 11845—11859

Figure 3. CAP mitigated DOX-induced ferroptosis in mice. (A) The cardiac tissues of mice from each group were stained with anti - 4-HNE (left) and quantitative analysis was performed (right). Scale: 50 µM (B) Mouse myocardial MDA (left), myocardial glutathione to reduced glutathione ratio (middle), myocardial iron non-heme iron (right) (n − 5). (C) Cluster heat maps of genes associated with ferroptosis in dox and CAP+DOX groups (n = 3). (D) mRNA expression levels of GPX4 (left) and PTGS2 (right) in mouse myocardium (n = 6). Data are shown as the mean ± SEM. Statistical significance was determined using two-tailed student’s t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.