Research Paper Volume 15, Issue 20 pp 11471—11488
The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study
Figure 7. Confirmation of the GSK3β/β-catenin/TERT pathway in BMSCs. (A, B) The siRNA-mediated depletion of GSK3β was assessed by western blotting. (C–E) The specific proteins related to the pathway protein in this experiment expressed from the NC group and siGSK3β group of BMSCs were detected by Western blotting, including β-catenin and TERT. (F–H) The siRNA-mediated depletion of β-catenin and protein levels of TERT was assessed by western blotting from the NC group and siβ-catenin of BMSCs. (I) Immunofluorescence staining reveals nuclear translocation of β-catenin in BMSCs after depletion of GSK3β. (J) Immunofluorescence assays were performed to identify colocalization between β-catenin and TERT in BMSCs after depletion of GSK3β. All results were performed as mean ± SD. Scale bar: 100 μm. #P < 0.05, ##P < 0.01 vs. NC group, n = 6.