Research Paper Volume 15, Issue 20 pp 11471—11488
The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study
Figure 4. LiCl reverses H2O2-induced inactivation of the Wnt pathway and inhibition of osteogenic differentiation in BMSCs. (A) CCK-8 analysis was conducted to evaluate cell viability after being treated with different concentrations of H2O2 for 6 h. (B) ALP staining in three groups of BMSCs. (C) ALP activity of BMSCs detected by ALP activity assay kit. (D–K) Protein expression levels of TERT, β-catenin, p-GSK3β/GSK3β, OPN, Runx2, BMP2 in BMSCs treated with H2O2 or LiCl by Western blotting. All results are performed as mean ± SD. #P < 0.05 vs. Control group. *P < 0.05, **P < 0.01, ***P < 0.005 vs. H2O2 group, n = 6.