Figure 9. Analyzing the co-occurrence of PGAM1 and CD163 in tumor biopsies during KIRC development. (A) Merge indicates PGAM1/CD163/DAPI; inset indicates local magnification; 2.5D reconstructed image shows the local fluorescence changes, morphology and fluorescence intensity. The merge image displays PGAM1/CD163/DAPI, while the inset exhibits a local magnification. The 2.5D reconstructed image showcases local fluorescence changes, morphology, and fluorescence intensity. (B) The Pearson's correlation coefficient was employed to visualize the degree of overlap between PGAM1 and CD163 fluorescence signals.