Research Paper Volume 15, Issue 19 pp 10732—10745

Figure 2. Micheliolide attenuated OVX induced excessive osteoclast bone resorption without affecting bone formation. Fifteen wild type female mice were randomly split into 3 groups (Sham group, OVX group and MCL group) as described in Figure 1. (A) Representative images of the plastic section TRAP staining pictures. Red arrows demonstrate stained TRAP positive osteoclasts. Scale bar = 50um. (B) Representative pictures of calcein (green) and alizarin red (red) labels were visualized. Scale bar = 50um. (C, D) The OcS/BS and N.Oc/BS were measured by TRAP staining sections. (E, F) N.Ob/SB and ObS/BS were measured by Toluidine Blue staining sections. (G, H) BFR/BS and MAR were measured by undecalcified sections. (I, J) The serum level of CTX1 and P1NP were evaluated. All data: mean ± SD, n=5, *p < 0.05 comparing to Sham group, **p < 0.01 comparing to Sham group, # p < 0.05 comparing to Sham group, ## p < 0.01 comparing to Sham group, N.S. no significant difference between each group.