Research Paper Volume 15, Issue 20 pp 10915—10937

Figure 6. Gcn5 is necessary for UDPG and Acetyl-CoA synthesis and H3K9 acetylation in glucose-depleted cells. (A) Global H3K9 acetylation levels in WT and gcn5Δ cells; (B–D) H3K9 acetylation levels in promoters of FBP1 (B), ACS1 (C) and PCK1 (D). (E, F) Relative levels of the TCA cycle metabolites (E) and Acetyl-CoA, UDPG, G1P and G6P together, and PEP (F) measured using LC-MS/MS. Student’s t-test was performed to reveal the differences between WT and gcn5Δ mutants or between EXP and PDS phases. ***: p < 0.001, **: 0.001<p< 0.01, *: 0.01<p< 0.05 and n.s.: p> 0.05. Error bars represent standard deviation calculated from biological triplicates. Abbreviation: EXP: exponential phase, PDS: early post-diauxic shift phase; OAA: oxaloacetate; UDPG: UDP-Glucose; G1P: Glucose-1-phosphate; G6P: Glucose-6-phosphate; PEP: Phosphoenolpyruvate.