Research Paper Volume 15, Issue 20 pp 10915—10937

Figure 2. Gcn5 functions within the HAT module of the SAGA/SLIK complex to promote starvation-induced gene expression and respiratory growth. (A, B) Relative expression levels of pSSA3-VFP (A) and pHSP26-VFP (B) in mutants of the SAGA/SLIK complexes; (C) Spotting assays of cell growth on YPD (2% glucose), YPG (3% glycerol) and YPE (1% Ethanol) media. HAT, histone acetyltransferase module; DUB, deubiquitinase module; Core, Core module; SLIK, SAGA-like complex. Expression levels of the two reporters in WT cells at 5.5h post-inoculation was set to 1. Error bars represent standard deviation calculated from biological triplicates. The significance of difference between WT and mutants across all time points was revealed by two-factor ANOVA analysis. ***: p < 0.001, **: 0.001<p< 0.01, *: 0.01<p< 0.05 and n.s.: p> 0.05.