Research Paper Volume 15, Issue 20 pp 11114—11130
Naked cuticle homolog 1 prevents mouse pulmonary arterial hypertension via inhibition of Wnt/β-catenin and oxidative stress
Figure 4. NKD1 inhibits hypoxia-induced proliferation and migration of PASMCs via reducing β-catenin expression. Hypoxia-challenged PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured with or without SKL2001 treatment. (A) Above treated PASMCs were stained with Ki-67 (green) and DAPI (blue). Representative pictures and the corresponding percentage of Ki-67-positive PASMCs were shown (post hoc for LSD test; n = 7 samples). Bar = 50 μm. (B) Migration of above-treated PASMCs were assessed by wound-healing assay (post hoc for LSD test; n = 6 samples). Bar = 200 μm. Data were shown as mean ± S.D. **P < 0.01 and ***P < 0.001 denoted statistical comparison between the two marked groups, respectively.