Research Paper Volume 15, Issue 20 pp 11114—11130

Figure 3. Downregulated β-catenin is responsible for NKD1-mediated regulation of oxidative stress in hypoxia-treated PASMCs. (A) PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured in the condition of normoxia or hypoxia. The relative expression of β-catenin in above-treated PASMCs was assessed by western blotting (post hoc for Dunnett’s T3 test; n = 5 samples). Hypoxia-challenged PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured with or without SKL2001 treatment. (B) The relative expression of β-catenin in above-treated PASMCs was assessed by western blotting (post hoc for LSD test; n = 6 samples). (C) Above treated PASMCs were stained with DHE. Representative pictures and the corresponding quantification of DHE fluorescence intensity were shown (post hoc for LSD test; n = 6 samples). Bar = 50 μm. Data were shown as mean ± S.D. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 denoted statistical comparison between the two marked groups, respectively.