Research Paper Volume 15, Issue 20 pp 11114—11130

Figure 1. NKD1 represses the proliferation and migration in hypoxia-treated PASMC. (A) The relative protein expression of NKD1 was assessed by western blotting in PASMCs after 12 h of hypoxia exposure (post hoc for LSD test; n = 6 samples). PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured in the condition of normoxia or hypoxia. (B) Above treated PASMCs were stained with Ki-67 (green) and DAPI (blue). Representative pictures and the corresponding percentage of Ki-67-positive PASMCs were shown (post hoc for LSD test; n = 7 samples). Bar = 50 μm. (C) Migration of above-treated PASMCs were assessed by wound-healing assay (post hoc for LSD test; n = 6 samples). Bar = 200 μm. Data were shown as mean ± S.D. ^**P < 0.01 and ^***P < 0.001 denoted statistical comparison between the two marked groups, respectively.