Research Paper Volume 15, Issue 19 pp 10627—10639

Figure 2. Progression of apoptotic cell after ischemia and reperfusion. (A) Representative images of immunofluorescence staining and (B) quantification of the TUNEL positive cardiomyocytes in mouse hearts treated as indicated (n = 4 : 4 : 4 : 4) Scale bar: 100 μm. (C) Representative western blot and (D, E) statistical data of myocardium apoptosis by detection of Bax, Bcl2, and Cleaved-Caspase 3 expression levels in mouse hearts treated as indicated (n = 3 : 3 : 3 : 3). MI=Myocardial infarction, Rep=reperfusion. Data are shown as mean ± SD. One-way ANOVA followed by Tukey post hoc test was used for statistical comparisons between multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001.