Research Paper Volume 15, Issue 19 pp 9965—9983
Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging
Figure 7. Autophagy contributes to cells’ survival during chronological aging. Simplified hypothetical model of diploid cell aging showing (1) activation of nucleophagy at the time of the steepest drop in cells survival during CLS experiment and (2) activation of PMN allowing removal of damaged DNA from the nucleus, likely preventing DNA rearrangements. The unusual phenotype of temporal accumulation of cells with propidium iodide-labeled buds is encompassed in the scheme. This scheme also underlines that cellular DNA content is gradually lost with time during chronological aging, up to the haploid level. Still, only diploid cells can regrow. PMN - piecemeal microautophagy of nucleus; PI - propidium iodide-stained cells.