Research Paper Volume 15, Issue 19 pp 10593—10606
UCHL1-PKM2 axis dysregulation is associated with promoted proliferation and invasiveness of urothelial bladder cancer cells
Figure 6. UCHL1 affects proliferation and migration capacity of UBC cells through PKM2 regulation. (A, B) Design and functional validation of PKM2 specific siRNA and overexpression vectors in UBC cancer cell line (T24 and UM-UC-3) via WB/PCR method. (C–E) CCK8 assay on UBC cell line groups respectively transfected with UCHL1 shRNA/overexpression vector in combination of PKM2 overexpression/siRNAs (n = 3). (F) Colony formation assay on UBC cell line groups respectively transfected with UCHL1 shRNAs/overexpression vector alone or in combination of PKM2 siRNA/overexpression vectors (n = 3). (G–I) Transwell and cellular invasion assay on UBC cell line groups transfected with UCHL1 shRNAs/overexpression alone or in combination with PKM2 siRNAs/overexpression vectors (n = 3). (J, K) Wound healing experiments on UBC cell line groups transfected with UCHL1 shRNAs/overexpression vector alone or in combination of PKM2 overexpression/siRNAs (n = 3). (L, M) WB investigation on EMT biomarkers and proliferation biomarkers in UBC cell line groups transfected UCHL1 shRNAs/overexpression vector alone or in combination of PKM2 overexpression/siRNAs.