Research Paper Volume 15, Issue 19 pp 10593—10606
UCHL1-PKM2 axis dysregulation is associated with promoted proliferation and invasiveness of urothelial bladder cancer cells
Figure 3. UCHL1 promotes the motility capacity of UBC cells in vitro. (A–D) Wound healing assay to investigate the influences of UCHL1 expression modulation on UBC tumor cell motility. Relative migration ratio of each cell group was statistically compared in bar charts (n = 3). (E–H) Transwell and cellular invasion assay to detect the impact of UCHL1 modulation on UBC tumor cell migration and invasion capabilities. Relative number of migrated or invaded cells of each group was statistically compared in bar charts (n = 3). (I, J) WB/qRT-PCR quantification of EMT biomarkers (E-cadherin, N-cadherin, Vimentin) in UBC cell groups transfected with UCHL1 shRNAs or overexpression vectors (n = 3).