Research Paper Volume 15, Issue 19 pp 10407—10427

Figure 2. PLCL1 inhibits RCC cancer cell migration and invasion. (A, B) Representative RT–qPCR analysis of 786-O and ACHN cells transfected with lentiviral vector encoding PLCLA and lentivirus vector. (C) Representative RT–qPCR analysis of 769P cells transfected with PLCL1 siRNA or scrambled control. (D–F) CCK8 assays were utilized to determine the proliferation of 786-O, ACHN and 769P cell lines. (G–I) Wound healing assays were used to explore the role of PLCL1 in RCC cells, and quantification analyses of the results were performed at 0 and 24 hours. Scale bar: 100 μm. (J–L) 786-O, ACHN and 769P cell lines with different treatments were examined by transwell assay. Scale bar: 100 μm. Data are shown as the mean ± SE from three independent experiments. Student’s t test was performed to determine statistical significance between two groups. Scale bar: 100 μm. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.