Research Paper Volume 15, Issue 19 pp 10291—10306
Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy
Figure 5. DOP elevated mitophagy in MPC5 cells. MPC5 cells were divided into 3 groups: normal glucose (5.5mM), HG (30mM) and HG with DOP treatment. (A) MPC5 cell viability of the three groups was detected by CCK-8 assay. (B) Flow cytometry was conducted to observe the mitochondrial membrane potential changes using JC-1 staining. (C, D) Detection of ROS content by using flow cytometry. (E) The expression of mitophagy-related proteins (Parkin, PINK1, LC3B) and mitophagy receptor P62 were detected by western blot. (F) Fluorescence intensity of mitochondrial MtphagyTracker and LysoTracker. The bar in the figure indicates 8 μm. (** p < 0.01; *** p < 0.001; n=3).