Research Paper Volume 15, Issue 19 pp 10291—10306
Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy
Figure 4. HG stimulation impaired mitochondrial function in MPC5 cells. MPC5 cells were divided into 5.5mm D-glucose group and 30mM D-glucose group. (A) Viability of MPC5 cells was detected by CCK-8 assay. From 12 h after stimulation, cell viability in the 30mM D-glucose group was significantly lower than that in the 5.5mm D-glucose group. (B) Mitochondrial membrane potential was evaluated by JC-1 staining assay. The percentage of mitochondrial membrane potential (Δψm) decreased is the percentage of JC-1 monomer. (C) DCF fluorescence was used to detect ROS levels. (*** p < 0.001; n=3).