Research Paper Volume 15, Issue 19 pp 10193—10212

Figure 1. Identification and confirmation of the OD of rat BMSCs. (A) BMSCs were divided into uninduced and osteogenic induced groups. The relative mRNA expression level of CK1ε was detected by qRT-PCR analysis after 7 and 14 days of incubation. (B) ARS of BMSC osteoblasts after two weeks of incubation. (C) The relative mRNA expression levels of OCN, RUNX2, SP7, and COL1A1 were detected by qRT-PCR after two weeks of incubation. (D) Qualitative analysis for ARS, and intracellular calcium concentration were detected after two weeks of induction. (E) Relative protein expression levels and semi-quantitative analysis of SP7, RUNX2, OCN, and COL1A1 after two weeks of incubation. The experiments in this figure were repeated three times, and similar results were obtained. Student’s t-test was used for (A, C, D, E). The data are presented as the means ± SD of independent experiments. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; Abbreviation: ns: not statistically significant.